MET Synthesis: Acid degradation of glycol chitosan, palmitoylation of acid degraded glycol chitosan with palmitic acid N-hydroxysuccinimide and alkylation using methyl iodide2.
MET Characterization: Level of palmitolyation and quaternisation was determined by 1H-NMR. Molecular weight was measured using GPC−MALLS. Residual solvent levels were determined by HPLC-UV detection and GC-FID.
LENK Analysis: peptide was quantified by HPLC-UV detection.
Nanoparticles Preparation: MET-LENK nanoparticles were prepared from LENK and MET in distilled water at a total solid concentration of 10% w/v and a MET:LENK ratio of 1 g/g followed by high pressure homogenization (1 cycle at 18000 Psi).
Nanoparticle Characterization: Nanoparticles mean diameter, polydispersity index and surface charge were determined in a Malvern Nano ZS. Morphology was evaluated by TEM microscopy.
Microparticle Preparation: Nanoparticles were spray dried in a Büchi Nano Spray Dryer B290 equipped with ultrasonic nozzle.
Microparticle characterization: Microparticle sizing was via a Mastersizer 3000. Morphology was evaluated by SEM microscopy.